Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots
Thermo Scientific Spectra Multicolor High Range Protein Ladder is a mixture of eight blue-, green-, and orange-stained proteins (40 to 300 kDa) for use as size standards for high-MW proteins in gel electrophoresis and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Applications
• Monitoring of protein migration during SDS-PAGE
• Verifying western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and western blots
• Locating a protein of interest for excision from an unstained preparative gel
For reverse transcription the kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT that maintains activity at 42 50°C and is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.
The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require a poly(A) tail, so can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.
Thermo Scientific Unstained Protein Molecular Weight Marker is a mixture of seven native proteins (14.4 to 116 kDa) for use as size standards in protein electrophoresis (SDS-PAGE).
Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots
• Thermostable—half life is more than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labeling
Product features
Reference bands—50 kDa band is stronger than the others for easy orientation
Tagged—the ladder proteins contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II Sequence